字級大小SCRIPT，如您的瀏覽器不支援，IE6請利用鍵盤按住ALT鍵 + V → X → (G)最大(L)較大(M)中(S)較小(A)小，來選擇適合您的文字大小，如為IE7或Firefoxy瀏覽器則可利用鍵盤 Ctrl + (+)放大 (-)縮小來改變字型大小。 Herpes simplex virus Type I (HSV-1) belonging to the Herpesviridae family of large enveloped DNA viruses is capable of causing a wide array of human ocular diseases. Herpes simplex keratitis (HSK)-induced cytopathogenicity together with the chronic immune-inflammatory reaction can trigger stromal scarring, thinning, and neovascularization which may lead to permanent vision impairment. Lychee flower extract is known for antioxidant and anti-inflammatory effects. Therefore, this study investigated the mechanism of SIRC cells infected by HSV-1 and examined the antiviral capabilities of lychee flower. SIRC cells were pretreated with Lychee flower extract (0.2 μg/ml、0.1 μg/ml、0.05 μg/ml) for 1.5 hours then infected with 1 M.O.I of HSV-1 for 24 hours. The supernatants and cell extracts were collected for Lactic dehydrogenase (LDH) assay, Cell Counting Kit-8 (CCK), plaque assay, Enzyme-linked immunosorbent assay (ELISA) and western blotting. The results indicate lychee flower extract decreases the cell cytotoxicity, cell viability, and virus titers; it also increases the IL-6 production for healing wounded cornea. In addition, lychee flower extract decreased JAK-2 protein expression causing anti-angiogenesis; mTOR pathway (pmTOR and pp70s6k) was inhibited the cell proliferation and enhanced the autophagy protein expression (Beclin-1 and LC3). The results showed the antiviral capabilities and the protective effects of lychee flower. According to our results suggests that lychee flower extract is potential an anti-HSK (herpes simplex keratitis) for HSV-1 infection.

目次

摘要 I
Abstract II
目次 III
圖目次 VI

第一章　緒言 1
第二章　文獻探討 2
第一節　單純疱疹病毒(herpes simplex virus)之簡介 2
第二節　單純疱疹病毒角膜炎(herpes simplex keratitis) 4
第三節　炎症反應及相關調控機制 5
第四節　細胞自噬 (Autophagy) 9
第五節　荔枝與荔枝花(Lychee and Lychee flower) 10
第三章　材料與方法 12
第一節　病毒增殖培養與純化 12
第二節　細胞的繼代與培養 14
第三節　SIRC細胞藥物前處理 15
第四節　乳酸脫氫酶LDH測定 (Lactic dehydrogenase assay) 16
第五節　細胞存活率測定 (Cell Counting Kit-8；CCK-8) 17
第六節　酵素免疫分析 (Enzyme-Linked Immunosorbent Assay, ELISA) 17
第七節　西方墨點法分析 (Western blotting analysi 17
第四章　實驗結果 20



第一節　探討第一型普通疱疹病毒(HSV-1)感染SIRC細胞的細胞型態 20
第二節　利用乳酸脫氫酶檢測探討低力價以及高力價第一型疱疹病毒(HSV-1)感染SIRC細胞後對細胞所造成的細胞毒性 20
第三節　利用存活率分析探討低力價以及高力價第一型疱疹病毒(HSV-1)感染SIRC細胞後之細胞存活率 20
第四節　利用ELISA測定HSV-1感染SIRC細胞後之促發炎細胞激素(pro-inflammatory cytokines)的變化 21
第五節　觀察不同濃度之荔枝花萃取物對於HSV-1感染SIRC細胞後之細胞型態變化 21
第六節　利用乳酸脫氫酶檢測不同劑量荔枝花萃取物之細胞毒性分析以及預先處理不同劑量荔枝花再以HSV-1 (1 M.O.I)感染SIRC細胞後之細胞毒性分析 21
第七節　探討不同劑量荔枝花萃取物以及預先處理不同劑量荔枝花再以HSV-1 (1 M.O.I)感染SIRC細胞後之細胞存活率 22
第八節　利用ELISA測定不同劑量荔枝花萃取物以及預先處理不同劑量荔枝花再以HSV-1 (1 M.O.I)感染SIRC細胞後之促發炎細胞激素(pro-inflammatory cytokines)的變化 23
第九節　利用病毒斑試驗測定預先前處理荔枝花萃取物對於HSV-1病毒力價的影響 23
第十節　探討荔枝花萃取物對於HSV-1感染SIRC細胞後所誘導的發炎性蛋白NF-κB p65、Phosphate NF-κB p65的表現量之影響 23



第十一節　探討荔枝花萃取物對於HSV-1感染SIRC細胞後對於JAK/STAT路徑蛋白表現量的影響 24
第十二節　探討荔枝花萃取物對於HSV-1感染SIRC細胞後對於pmTOR、pp70s6k、ps6蛋白表現量的影響 25
第十三節　探討荔枝花萃取物對於HSV-1感染SIRC細胞後對於Beclin-1、LC3蛋白表現量的影響 25
第五章　討論 27
圖表 31
附錄 44
參考文獻 50





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